KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Myc tag | Cell Signaling | Cat# 2276S; RRID:AB_331783 |
| Rabbit polyclonal anti-human TRF2 | Novus | Cat# NB110-57130; RRID:AB_844199 |
| Mouse monoclonal anti-human SNF2H | Santa Cruz | Cat# sc-365727; RRID:AB_10844618 |
| Goat biotinylated anti-avidin | Vector | Cat#BA-0300; RRID:AB_2336108 |
| Mouse monoclonal anti-BrdU(IdU) | BD | Cat# 347580; RRID:AB_400326 |
| Rat monoclonal anti-BrdU(CldU) | Accurate | Cat# OBT0030; RRID:AB_2313756 |
| Rabbit polyclonal anti-actin | Sigma | Cat#A2066; RRID:AB_476693 |
| Goat polyclonal anti-Mouse IgG Alexa Fluor 568 | ThermoFisher | Cat#A11031; RRID:AB_144696 |
| Goat polyclonal anti-Rat IgG Alexa Fluor 488 | ThermoFisher | Cat#A11006; RRID:AB_2534074 |
| Goat polyclonal anti-Rabbit IRDye800CW | Li-Cor | Cat#926-32211; RRID:AB_621843 |
| Goat polyclonal anti-Mouse IRDye 680LT | Li-Cor | Cat#926-68020; RRID:AB_10706161 |
| Rabbit polyclonal anti-ORC2 | Bethyl | Cat# A302-735A: RRID:AB_10627808 |
| Rabbit polyclonal anti-MCM3 | Abcam | Cat#ab4460; RRID:AB_304469 |
| Rabbit polyclonal anti-H3K9me3 | Diagenode | Cat#C15410193; RRID:AB_2616044 |
| Rabbit polyclonal anti-TRF2 | Deng et al., 2009 | N/A |
| Normal rabbit IgG | Cell Signaling | Cat#2729S; RRID:AB_1031062 |
| Bacterial and Virus Strains | ||
| Escherichia coli Stable competent cells | NEB | Cat#C3040H |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Doxycycline hyclate | Sigma | Cat#D9891 |
| Puromycin | Sigma | Cat#P8833 |
| Aphidicolin | Sigma | Cat# A0781 |
| Q5 Hot Start High-Fidelity DNA Polymerase | NEB | Cat#M0493 |
| XtremeGene HP transfection reagent | Roche | Cat#06 366 244 001 |
| Alexa Fluor 350–conjugated NeutrAvidin | Invitrogen | Cat# A11236 |
| Colcemid | GIBCO | Cat# |
| Blocking Reagent | Roche | Cat#11096176001 |
| Blotting-grade Blocker | BioRad | Cat# 1706404 |
| Critical Commercial Assays | ||
| QIAquick PCR Purification Kit | QIAGEN | Cat#28104 |
| QIAquick Gel Extraction Kit | QIAGEN | Cat#28704 |
| Wizard Genomic DNA Purification Kit | Promega | Cat# A1120 |
| Experimental Models: Cell Lines | ||
| LOX human melanoma cells | Laboratory of Lifeng Xu | RRID:CVCL_1381 |
| Oligonucleotides | ||
| Primer: shRNA Forward: ACGTGCGGCCGCTCCAGCCCTCACTCCTTCTC | This paper | N/A |
| Primer: shRNA Reverse: CAAAGAACGGAGCCGGTTGG | This paper | N/A |
| Biotin-OO-(CCCTAA)4 PNA | BioSythesis | N/A |
| Cy3-(CCCTAA)3 PNA | PNA Bio | N/A |
| Recombinant DNA | ||
| pLPC-NMYC TRF2 | Smogorzewska and de Lange, 2002 | Addgene Plasmid # 16066; RRID:Addgene_16066 |
| pLPC-NMYC TRF2deltaB | Smogorzewska and de Lange, 2002 | Addgene Plasmid #16067; RRID:Addgene_16067 |
| pInducer10 | Meerbrey et al., 2011 | Addgene Plasmid #44011; RRID:Addgene_44011 |
| pInducer10L | Drosopoulos et al., 2020 | N/A |
| pIND-MYC-TRF2 | This paper | N/A |
| pIND-MYC-TRF2ΔB | This paper | N/A |
| pIndSNF2HshmiR | This paper | N/A |
| pIndLucshmiR | This paper | N/A |
| Software and Algorithms | ||
| ImageStudio | Li-Cor | https://www.licor.com/bio/image-studio/ |
| ImageJ | NIH | https://imagej.nih.gov |
| ImageQuant TL | GE Healthcare | http://www.cytivalifesciences.com/country-selection?originalItemPath=%2f |
| ImagePro Plus | Media Cybernetics | https://www.mediacy.com/imageproplus |
| NIS Elements Advanced Research | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| Excel | Microsoft | https://www.microsoft.com/en-us/microsoft-365?rtc=1 |
| Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Photoshop | Adobe | https://www.adobe.com/products/photoshop.html |
| Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |