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. Author manuscript; available in PMC: 2021 May 24.
Published in final edited form as: Nat Immunol. 2020 Nov 24;22(1):86–98. doi: 10.1038/s41590-020-00835-8

Extended Data Fig. 4. Analysis of temporal host RNA and virus-specific probe expression in SARS-CoV-2-infected AGM BAL and blood samples.

Extended Data Fig. 4

Principal Component Analyses (PCA) indicate overall sample variance in a) BAL and b) blood AGM transcriptomes when filtered by day post infection (dpi) and are shown to highlight time-dependent host transcriptional changes. PC1 (principal component 1), PC2 (principal component 2). c) At the indicated time point, the expression of individual virus-specific probes in BAL samples of each subject is plotted. Data are presented as mean values +/− SD. Statistics were derived from n=6 biologically independent animals/samples for pre-, 3, and 5 dpi time points and n=3 biologically independent animals/samples for the 7 dpi time point in a single experiment. A mixed effects model with Geisser-Greenhouse correction and a Tukey’s multi-comparisons test revealed no statistical significance between individual probes at any particular time point, but significant overall time-dependent effects (p=0.0003).