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. Author manuscript; available in PMC: 2021 May 24.
Published in final edited form as: Nat Immunol. 2020 Nov 24;22(1):86–98. doi: 10.1038/s41590-020-00835-8

Extended Data Fig. 2. Comparison of viral loads of tissues from primary and re-challenged AGMs.

Extended Data Fig. 2

Tissues harvested at necropsy from SARS-CoV-2 infected-AGMs (n=6) were processed to determine viral loads by a) RT-qPCR and b) plaque titration. Tissues from animals sacrificed at 5 dpi (n=3) were compared to those re-challenged at 35 dpi and sacrificed at 57/22 dpi (n=3). Abbreviations for tissues: ALN: Axillary lymph node, ILN: inguinal lymph node, Liv: liver, Spl: spleen, Kid: kidney, Adr: adrenal gland, RUL: right upper lung, RML: right middle lung, RLL: right lower lung, LUL: left upper lung, LML: left middle lung, LLL: left lower lung, BFC: brain frontal cortex, BS: brain stem, CSC: cervical spinal cord, MLN: mandibular lymph node, smLN: submandibular lymph node, Ton: tonsil, Trac: trachea, Hrt: heart, MsLN: mesenteric lymph node, Sto: stomach, Duo: duodenum, Pan: pancreas, Ile: ileum, IleJxn: ileocecal junction, TC: transverse colon, UB: urinary bladder, Gon: gonad, Ut/Pros: uterus/prostate, NaMu: nasal mucosa, Conj: conjunctiva. The horizontal dashed line indicates the LOD for the assay. Multiple two-tailed t-tests using the Bonferroni-Dunn method: p= 0.0332 (*), 0.0021 (**), 0.0002 (***), <0.0001 (****). Data are presented as mean values +/− SEM. Statistics were derived from the mean of duplicate RT-qPCR reactions or wells of each tissue per animal (n=6 biologically independent animals/samples per tissue in a single experiment; n=3 animals per cohort (5 or 57/22 dpi).