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. Author manuscript; available in PMC: 2021 Jan 7.
Published in final edited form as: Cell Rep. 2020 Dec 22;33(12):108527. doi: 10.1016/j.celrep.2020.108527

Figure 3. Accumulation of tRNA Fragments and Operon Activation Occur via Two Path- ways.

Figure 3.

(A) RNA from strains grown to mid-log or stationary phase was subjected to northern blotting to detect tRNATrp(CCA) 5′ halves. Lane 1, size markers (nt).

(B) Immunoblots were performed on lysates from the indicated FLAG3-rsr strains to detect FLAG3-Rsr. RplE, loading control.

(C and D). RNA from the indicated strains grown to stationary phase was subjected to northern blotting to detect 5′ halves of tRNAHis(GUG) (C) and tRNA-Leu(UAG) (D).

(E–G) Lysates from the indicated strains were immunoblotted to detect FLAG3-Rsr. RplE, loading control.

In (A), (C), and (D), red lines denote fragments differing in mobility or levels in mutant strains.