Figure 5.

Expression of CD39 and CD73 on activated in vitro CD8⁺ T cells and CD4⁺FoxP3⁻ Tcon. Splenocytes from healthy mice were stimulated with plate-bound anti-CD3 and anti-CD28 mAbs for 6 h, 12 h, 24 h, 48 h, and 72 h (A-D) or for 12 h (E, F). The expression of CD39 and CD73 on activated (a-CD3, a-CD28) and non-activated (unstim) CD8⁺ T cells (A, B, E, F) and CD4⁺FoxP3⁻ Tcon (C, D) was analyzed by flow cytometry. Results are shown as the percentage of CD39⁺ or CD73⁺ cells within indicated T cell subsets (n = 6–10). *P < .05, **P < .01, ***P < .001, ****P < .0001