Figure 6.

Ectonucleotidase expression on PD-1⁺ T cells from melanoma-bearing hosts. Expression of CD39, CD69, and PD-1 was measured in CD8⁺ T cells (A, B) or CD4⁺FoxP3⁺ Tcon cells (C, D) from skin tumors of RET-tg mice (n = 6) by flow cytometry. The results are shown as the percentage of CD39⁺ cells within total PD-1⁺ or PD-1⁻ T cells (A, C) or within PD-1⁺CD69⁺ or PD-1⁻CD69⁺ subsets (B, D). Expression of CD39, CD73 and PD-1 was tested in total CD8⁺ T cells (E, F) or CD4⁺FOXP3⁻CD25^low/-CD127^high Tcon (G, H) from the peripheral blood of melanoma patients (n = 18) and healthy donors (n = 6). The results are presented as the percentage of CD39⁺ and CD73⁺ cells among PD-1⁺ or PD-1⁻ indicated subsets of T cells. *P < .05, ***P < .001