Figure 8.

Tumor soluble factors induce ectonucleotidase expression on CD8⁺ T cells. Normal splenocytes were cultivated for 48 h in presence of Panc02 conditioned medium diluted 1:4 or 1:2. In some wells, anti-TGF-β antibodies (10 μg/ml) were added. Expression of CD39 and CD73 on various T cell subsets was analyzed by flow cytometry (n = 12–16). (A) shows representative dot plots. Ectonucleotidase expression is presented as the percentage of CD39⁺CD73⁺ cells within CD69⁺CD8⁺ T cells (b). The level of CD73 expression on CD73⁺CD39⁺CD8⁺ T cells is shown as mean fluorescence intensity (MFI), **P < .01, ***P < .001