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. 2020 Dec 31;2020:6694214. doi: 10.1155/2020/6694214

Figure 3.

Figure 3

Bergenin decreases autophagy by downregulating Beclin-1 and LC-3. (a) mRNA expression of Beclin-1 and LC3-II assessed by real-time PCR (n = 8). (b) Protein expression of Beclin-1 and LC3-II assessed by western blotting. The quantitative evaluation was determined by relative band density. (c) Immunohistochemical staining of Beclin-1 and LC3-II (original magnification = 200x). (d) The amount of autophagosome significantly decreased as showed by TEM (original magnification: ×10000). ∗p < 0.05 for CCl4/BDL vs. sham, #p < 0.05 for CCl4/BDL+B20 vs. CCl4/BDL, and ^p < 0.05 for CCl4/BDL+B40 vs. CCl4/BDL+B20.