Figure 2.

DOCK8 is not required for deletion of strongly self‐reactive CD4SP thymocytes. For thymocytes from mice of the indicated genotypes (top), plots show (a) expression of Foxp3 versus TCR^3A9 with numbers representing the percentage of cells in the adjacent gate, enumerated in the summaries (right). (b) CD4/CD8 phenotype of TCR^3A9+ Foxp3⁻ thymocytes with a summary showing the percentage of TCR^3A9+ Foxp3⁻ CD4SP cells among all thymocytes (right). TCR^3A9+ Foxp3⁻ CD4SP thymocytes were analysed for (c) Helios/CCR7 phenotype, with a gate for antigen‐inexperienced Helios⁻ CCR7⁺ cells, and (d) Helios/Bim phenotype, with a gate for antigen‐experienced Helios⁺ Bim⁺ cells, enumerated in the summaries (right). In a–d, each symbol in a summary graph represents a measurement from 1 mouse (n = 3 Dock8^+/+3A9⁺, n = 2 Dock8^pri/pri3A9⁺, n = 4 Dock8^+/+3A9⁺insHEL⁺ and n = 5 Dock8^pri/pri3A9⁺insHEL⁺), and horizontal bars show the group mean compiled from 2 experiments. (e) Mixed chimeras made by reconstituting irradiated insHEL⁻ or insHEL⁺ hosts with mixtures of CD45.1 wild‐type 3A9⁺ BM plus CD45.2 Dock8^+/+ or Dock8^pri/pri 3A9⁺ BM were analysed 8 weeks after BM transplantation. Plots show CD45.2⁺ TCR^3A9+ Foxp3⁻ CD4SP thymocytes analysed for Helios/CCR7 (top) or Helios/Bim (bottom) phenotypes, with summaries (right) showing the frequency of subsets gated on the plots. Each line joins frequencies for the CD45.1⁺ and CD45.2⁺ subsets of TCR^3A9+ Foxp3⁻ CD4SP thymocytes from an individual mouse (n = 4 CD45.1Dock8^+/+: CD45.2 Dock8^+/+ into insHEL⁻; n = 2 CD45.1Dock8^+/+: CD45.2 Dock8^pri/pri into insHEL⁻; n = 4 CD45.1Dock8^+/+: CD45.2 Dock8^+/+ into insHEL⁺; and n = 3 CD45.1Dock8^+/+: CD45.2 Dock8^pri/pri into insHEL⁺). Statistical analyses used 2‐way ANOVA with Sidak’s multiple comparisons test (a–d) or paired Student’s t‐tests (e); P‐values: * < 0.05, ** < 0.01 and **** < 0.0001.