Fig. 5.

Washout probabilities upon mis-regulated Wnt and Hippo signalling activity. (a, b) Washout probabilities for APC double-mutant crypt, starting from a single mutant cell introduced at the base of the crypt (bottom 5% of crypt) using (a) externally-imposed gradient (M_E1) or (b) cell-division based (M_I) Wnt models. Blue and red lines represent the low (40% Wnt threshold/10% Wnt reservoir) and high (100% Wnt threshold/20% Wnt reservoir) Wnt cases, respectively. Increasing the volume threshold for CI reduces the probability of mutant washout. Shaded regions represent 95% confidence intervals for a binomial distribution with probability $p$, according to $p\pm z_{0.975}\sqrt{p(1-p)/n}$, where $n$ is the number of simulations conducted, and $z_{0.975}$ is the 97.5 percentile of a standard normal distribution. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)