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. 2020 Oct 13;40(1):28–45. doi: 10.1038/s41388-020-01498-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The expression levels of IGF2BP1 in 374 HCC tissues and 50 adjacent nontumor liver tissues in the TCGA cohort were analyzed by a t-test. Data are shown after a log2 transformation. B The correlation between PTPN13 and IGF2BP1 expression in 374 HCC tissue samples. Correlations were then analyzed by Pearson’s correlation analysis. C The effects of transient PTPN13 overexpression and a control vector in PLC/PRF/5 and LM3 cells and PTPN13 knockdown with shRNA and a control shRNA in Huh7 and SMMC-7721 cells on the mRNA expression of IGF2BP1 downstream genes are shown. D Immunoblot analysis of genes downstream of IGF2BP1 and relevant pathways related to cancer proliferation and metastasis (c-Myc, MDR1, PTEN, p-AKT, and p-mTOR) in PLC/PRF/5 and LM3 cells with transient PTPN13 overexpression and a control vector and Huh7 and SMMC-7721 cells with knockdown and control shRNA. E A RIP assay was performed using anti-IGF2BP1 and control IgG antibodies after transient PTPN13 overexpression, followed by qRT-PCR to examine the enrichment of c-Myc, MDR1, IGF2, and GAPDH. IGF2 served as a positive control, while GAPDH served as a negative control. F Immunoblot analysis of c-Myc expression in HCC cells expressing PTPN13-specific shRNAs or control cells with or without the transient transfection of IGF2BP1-specific siRNAs. Data represent the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01; ns not significant).