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. 2020 May 26;14(1):26–37. doi: 10.1038/s41385-020-0298-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Inflammation (black arrows) in the lungs of bone marrow chimaeras was analysed in H&E stained, paraffin-embedded sections (×20 magnification, 20 μm scale bar). b Neutrophil numbers assessed by flow cytometry in the lung and spleen of bone marrow chimaeras. c, d Flow cytometry plots and graphed data showing analysis of SiglecF and Ly6G expression in bone marrow from chimaeras. Plots are gated on LiveCD45.2⁺CD45.1⁻CD11b⁺CD19⁻ cells. Open purple circle denotes a previously uncharacterised Ly6G^int bone marrow population shown in the graph (right). Wild-type B6SJL mice received C57BL/6 or Ear11Tg bone marrow (BM). Data representative of two independent experiments, n = 4–7.