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. 2020 May 26;14(1):26–37. doi: 10.1038/s41385-020-0298-2

Fig. 4. Ear11mCherry is expressed preferentially by alternatively activated macrophages.

Fig. 4

a Expression of Ear11, Retnla (Relma), and Arg1 by alveolar Ear11-negative or Ear11-positive macrophages sorted from Ear11+/Ch mice treated with intranasal IL-25. Assessed by qPCR. b Ear11+/Ch expression by peritoneal macrophages (CD45+CD11b+F4/80+) cultured in a time-course in either media alone or media supplemented with rmIL-25, rmIL-33, rmIL-13, or rmIL-4 at 10 ng/ml. Cells were imaged every 24 h using a Nikon HCA fluorescence microscope. c Ear11 expression by peritoneal macrophages cultured as indicated (in b) for 72 h, assessed by qPCR. Cells from 20 pooled mice, two wells/condition. d Ear11 protein concentration in cultures (as in b) assessed by ELISA. Data representative of two independent experiments. e Ear11 expression in sorted alveolar macrophages and f, g total lung neutrophil numbers as assessed by flow cytometry, following two consecutive intranasal doses of IL-25 or IL-25 and Ear11. Data pooled from two independent experiments (n = 4–6 per experiment).