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. 2020 May 26;14(1):26–37. doi: 10.1038/s41385-020-0298-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Flow cytometric analysis of total neutrophil numbers in a the lung and spleen, (data pooled from 3 independent experiments, n = 3–6 per experiment), blood (data pooled from three independent experiments, n = 10–20 per experiment), and b bone marrow (representative of two independent experiments, n = 6) of naive Ear11^+/+ and Ear11^Ch/Ch mice. c Total number of lung and spleen macrophages in naive Ear11^+/+ and Ear11^Ch/Ch mice. d Total number of lung neutrophils in mice treated intranasally with either PBS or IL-25. e Total number of lung macrophages in mice treated intranasally with PBS or RWP. f Total number of lung neutrophils in mice treated intranasally with either PBS or RWP. g Quantitative PCR analysis of Muc5ac and Muc5b in lung tissue from e. c–g Data representative of two independent experiments, n = 5–10 per experiment.