Fig. 1.

HNF4α-induced gastric mucosal changes in mice. a Schematics of the gene constructs for Lgr5-Cre mice and LSL-Hnf4α mice, and the experimental strategy for Cre-mediated recombination in the Rosa26^Hnf4α mice. b Representative electron microscopy pictures showing mucin of Rosa26^Hnf4α mice gastric epithelium (arrows). Scale bar: 2 μm. c Immunohistochemistry (IHC) for Hnf4α in the stomach section of WT and Rosa26^Hnf4α mice. The right boxes indicate regions enlarged. Scale bars: 200 µm (left); 50 µm (right). d HE staining for gastric sections of WT and Rosa26^Hnf4α mice. Scale bars: 100 µm. e Different sections from WT and Rosa26^Hnf4α mice were examined by Alcian blue staining–periodic acid–Schiff (AB–PAS) staining. Scale bars: 1000 µm (left); 50 µm (right)