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. 2021 Jan 4;12(1):35. doi: 10.1038/s41419-020-03330-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Schematic representing isolation of epidermal cells from human foreskin tissue. B Comparison of the viability of cells derived from the traditional method and the novel method. Results are the mean ± SD from five independent repeated experiments. n.s., not significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001. C Morphology of isolated human epidermal cells after attachment in a low-calcium, serum-free medium (EpiLife). D Immunofluorescence analysis of epidermal progenitor cell markers, CK14 and P63, in the attached epidermal cells. E Representative serial images of hPEOs growing at the indicated time points in the NaNBEFNoRWAFs medium. SD, standard deviation. Scale bar: 50 µm (C, D), 100 µm (E).