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. 2021 Jan 4;12(1):35. doi: 10.1038/s41419-020-03330-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A ITGA6 immunostaining of human primary epidermis tissue counterstained with DAPI. B Human epidermal cell suspensions were separated into ITGA6^high cells and ITGA6^low/− cells. Identity of the populations was confirmed by staining for CK14 and CK10. Sorted cells were grown in organoid cultures for 9 days. C Representative serial images of an epidermal organoid generated from single ITGA6^high cells. Scale bar: 50 µm (A), 100 µm (C).