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. 2021 Jan 7;12(1):22. doi: 10.1038/s41419-020-03272-5

Fig. 4. The reversal of CD4+ Treg suppressive function mediated by TLR8 signal may have a relationship with glucose metabolism.

Fig. 4

A, B The expression levels of glucose metabolism-related genes (Glut1, Glut3, GPI, TPI, HIF-1α, LDH-α, PKM2, Eno1) in Tregs treated with 2-DG and Galloflavin. Expression levels of each gene were normalized to β-actin expression level and adjusted to the levels in CD4+ Tregs without treatment (served as 1). Data shown are mean ± SEM; *P < 0.05; **P < 0.01. C The expression levels of glucose metabolism-related proteins (Glut1, GPI, LDH-α, PKM2) in Tregs treated with two inhibitors. The upper four panels show the western blot analysis results. The bottom panel shows the protein expressions analyzed quantitatively and compared with β-actin expression with a densitometer. Results shown in the histogram are mean ± SEM; *P < 0.05; **P < 0.01. D The proliferation levels of Teff cells co-cultured with different groups of Treg cells. Left images are the representative flow cytometric analysis of Teffs in different groups. Right bar diagram shows the proliferation ratio of Teffs. Data were displayed as mean ± SEM; *P < 0.05; ns P > 0.05.