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. 2021 Jan 7;12(1):10. doi: 10.1038/s41419-020-03278-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blotting analysis revealed that TRAF3 expression levels increased post-SAH. B Quantitative analysis of TRAF3/GAPDH. N = 6 mice per group. *p < 0.05 versus the sham group, ^#P < 0.05 versus the SAH 48 h group. C, D Western blotting analysis revealed that the expression of TRAF3 in SAH + 125 pmol of TRAF3 siRNA group showed no significant change compared with SAH + control siRNA group. However, 250 and 500 pmol of TRAF3 siRNA significantly reduced the TRAF3 expression. GAPDH served as a loading control. N = 6 mice per group. *p < 0.05 versus the SAH group. E Representative double immunofluorescence images showed that TRAF3 expression was elevated 24 h following SAH and was mainly expressed in neurons, with sparse expression in microglia and no expression in astrocytes. Scale bar: 50 μm. The TRAF3 positive index of neurons (F), microglia (G), and astrocytes (H). N = 6 mice per group. *p < 0.05 versus the sham group.