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. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Cell Metab. 2020 Nov 4;33(1):199–210.e8. doi: 10.1016/j.cmet.2020.10.018

Figure 1. Parallel CRISPR-Cas9 metabolism screens reveal metabolic dependencies in PDA.

Figure 1.

A, Schematic of parallel CRISPR/Cas9 screen design using a B6 KPC line. B, Gene score correlation between in vitro (passage 7, P7) and in vivo. C, Comparison of significantly depleted genes (FDR < 0.2) in the in vitro (P7) and in vivo screen. FDR is calculated using STARS algorithm. D, Pathway analysis of significantly depleted genes (FDR < 0.2) using Metaboanalyst. The –Log10 p-value of the most enriched pathways (FDR < 0.005) are represented. E, Differential dependencies are plotted by looking at the delta log fold change between in vivo and in vitro (P7) conditions. STARS score is plotted on the y axis and differentially required genes with an FDR < 0.02 are colored.