Major Resources Table

Animals (in vivo studies)
Species	Vendor or Source	Background Strain	Sex	Persistent ID / URL
NA	NA	NA	NA	NA

Genetically Modified Animals
	Species	Vendor or Source	Background Strain	Other Information	Persistent ID / URL
Pseudo-WT-α1C	Mouse	Transgenic Mouse Facility Columbia University	B6CBAF2	Generated at Columbia University	NA
GGG-α1C	Mouse	Transgenic Mouse Facility Columbia University	B6CBAF2	Generated at Columbia University	NA
AID-α1C	Mouse	Transgenic Mouse Facility Columbia University	B6CBAF2	Generated at Columbia University	NA
9*-α1C	Mouse	Transgenic Mouse Facility Columbia University	B6CBAF2	Generated at Columbia University	NA
FVB/N-Tg(Myh6-rtTA)8585Jam/Mmmh	Mouse	MMRC	FVB	NA	RRID: MMRRC_ 010478-MU

Antibodies
Target antigen	Vendor or Source	Catalog #	Working concentration	Lot # (preferred but not required)	Persistent ID / URL
FLAG (rabbit)	Sigma	F7425	1:200 (ICC)		https://www.sigmaaldrich.com/catalog/product/sigma/f7425
Monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody	Sigma	A8592	1:1000 (WB)		https://www.sigmaaldrich.com/catalog/product/sigma/a8592?lang=en&region=US
Beta common (custom)	Yen-Zym		1:1000		epitope: mouse residues 120–138:DSYTSRPS-DSDVSLEEDRE
AlexaFluor 594-goat anti rabbit	Thermo	A11037	1:200	2079421	https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11037

DNA/cDNA Clones
Clone Name	Sequence	Source / Repository	Persistent ID / URL
mouse Rrad mRNA		NCBI/GenBank	XM_006531206
human Xacnb2 mRNA		NCBI/GenBank	NM_201590.3
rabbit cardiac Cacna1c mRNA		NCBI/GenBank	X15539
rabbit smooth muscle Cacna1c mRNA		NCBI/GenBank	X55763

Cultured Cells
Name	Vendor or Source	Sex (F, M, or unknown)	Persistent ID / URL
HEK293T cells	ATCC		CRL-3216
HEK293 cells	ATCC		CRL-1573

Data & Code Availability
Description	Source / Repository	Persistent ID / URL

Other
Description	Source / Repository	Persistent ID / URL
polyethylenimine (PEI)	Polysciences	23966-2; https://www.polysciences.com/default/polyethylenimine-linear-mw25000
cycloheximide	Sigma	01810-1G; https://www.sigmaaldrich.com/catalog/product/sigma/c4859?lang=en&region=US
Trypsin 0.05%	Thermo Fisher	25300-054; https://www.thermofisher.com/order/catalog/product/25300054?SID=srch-hj-25300-054#/25300054?SID=srch-hj-25300-054
FBS	Thermo Fisher	10438-026; https://www.thermofisher.com/order/catalog/product/10438026?SID=srch-hj-10438-026#/10438026?SID=srch-hj-10438-026
DMEM	Thermo Fisher	11995-065; https://www.thermofisher.com/order/catalog/product/11995065?SID=srch-hj-11995-065#/11995065?SID=srch-hj-11995-065
Lipofectamine 2000	Thermo Fisher	11668-019; https://www.thermofisher.com/order/catalog/product/11668019?SID=srch-hj-11668-019#/11668019?SID=srch-hj-11668-019
Pen/Strep Glutamine	Thermo Fisher	10378-016; https://www.thermofisher.com/order/catalog/product/10378016?SID=srch-hj-10378-016#/10378016?SID=srch-hj-10378-016
optiMEM	Thermo Fisher	31985-062; https://www.thermofisher.com/order/catalog/product/31985062?SID=srch-hj-31985-062#/31985062?SID=srch-hj-31985-062
Attachment Factor	Thermo Fisher	S-006-100; https://www.thermofisher.com/order/catalog/product/S006100?SID=srch-hj-S-006-100#/S006100?SID=srch-hj-S-006-100
Doxycycline Rodent Diet (200 mg/kg)	Bio Serv	S3888; https://us.vwr.com/store/product/15984109/doxycycline-rodent-diet-sterile-bio-serv
Triton X-100	IBI Scientific	IB07100; https://www.ibisci.com/products/triton-x-100
Calpain inhibitor I	Sigma	A6185; https://www.sigmaaldrich.com/catalog/product/sigma/a6185
Calpain inhibitor II	Sigma	A6060; https://www.sigmaaldrich.com/catalog/product/sigma/a6060
Complete Mini tablets	Roche	04693159001; https://www.sigmaaldrich.com/catalog/product/roche/04693159001?lang=en&region=US
Protein A beads	GE Healthcare	17-0780-01; https://www.sigmaaldrich.com/catalog/product/sigma/ge17078001?lang=en&region=US
Forskolin	Santa Cruz Biotechnology	SC-7562; https://www.scbt.com/p/forskolin-66575-29-9
Isoproterenol	Sigma	I5627; https://www.sigmaaldrich.com/catalog/product/sigma/i5627
Nisoldipine	Santa Cruz Biotechnology	SC-212396; https://www.scbt.com/p/nisoldipine-d7
Amphotericin B	Sigma	A9528; https://www.sigmaaldrich.com/catalog/product/sigma/a9528?lang=en&region=US
SYBR Green qPCR Master Mix	Thermo	https://www.thermofisher.com/order/catalog/product/4309155#/4309155
RNeasy Mini kit	Qiagen	https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/rna-purification/total-rna/rneasy-mini-kit/#orderinginformation
High-Capacity cDNA RT kit	Thermo Scientific	https://www.thermofisher.com/order/catalog/product/4368814#/4368814
Red ANTI-FLAG M2 Affinity Gel	Sigma	F2426; https://www.sigmaaldrich.com/catalog/search?term=F2426&interface=All&N=0&mode=match%20partialmax&lang=en&region=US&focus=product
Liberase	Sigma	5401119001; https://www.sigmaaldrich.com/catalog/product/roche/libtmro?lang=en&region=US