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. Author manuscript; available in PMC: 2022 Jan 8.
Published in final edited form as: Circ Res. 2020 Nov 2;128(1):42–58. doi: 10.1161/CIRCRESAHA.120.317372

Fig. 1. Intracranial lymphatic vessels in the adult zebrafish.

Fig. 1

A. Dorsal view image of the head of an adult casper (roy, nacre double mutant) zebrafish. B. Schematic diagram of the boxed region in panel A, showing the optic tecta and cerebellum with a typical network of intracranial lymphatic vessels in a young adult zebrafish. C. Confocal image of the dorsal head of a Tg(mrc1a:egfp)y251, casper adult zebrafish, with mrc1a+FGPs and superficial lymphatics in grey and intracranial meningeal lymphatics pseudocolored green. D. Confocal image of the dorsal surface of a dissected brain from a Tg(−5.2lyve1b:DsRed)nz101, casper adult zebrafish, with lyve1+ FGPs but no lymphatic vessels. E. Schematic diagram of dissection of an adult zebrafish head for imaging the dorsal surface of the brain and the ventral surface of the skull. F. Confocal image of the ventral (inner) surface of a dissected brain from a Tg(−5.2lyve1b:DsRed)nz101, casper adult zebrafish, with lyve1+ lymphatic vessels but no FGPs. G. Higher magnification confocal image of the dorsal surface of a dissected brain removed from a Tg(−5.2lyve1b:DsRed)nz101, casper adult zebrafish, showing individual separated lyve1+ FGPs with characteristic large autofluorescent internal vacuoles. H. Higher magnification confocal image of the outer layer of the brain imaged through the skull of a living casper adult double transgenic zebrafish Tg(mrc1a:egfp)y251, Tg(kdrl:mcherry) y206, showing mrc1a+ lymphatic vessels and kdrl+ blood vessels. I. Higher magnification confocal image of the dorsal head of a Tg(mrc1a:egfp)y251, Tg(kdrl:mcherry) y206, casper adult zebrafish. This orthogonal view shows a cross section of an mrc1a+ lymphatic vessel immediately below the blue autofluorescent skull and an mrc1a+ FGP in a deeper layer immediately adjacent to a kdrl+ blood vessel. Unless otherwise noted all images are dorsal views, rostral to the left. Scale bars: 500 um (A,C,E,F) 25 um (G,H,I). (BV- blood vessels, roy- roy orbison, mrc1a-mannose receptor C, type 1a, eGFP- green fluorescent protein, Tg- transgenic, lyve1b- lymphatic vessel endothelial hyaluronic receptor 1b, FGP- fluorescent granular perithelial cells, kdrl-kinase insert domain receptor like)