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. Author manuscript; available in PMC: 2022 Jan 8.
Published in final edited form as: Circ Res. 2020 Nov 2;128(1):42–58. doi: 10.1161/CIRCRESAHA.120.317372

Fig. 3. Functional validation of zebrafish intracranial lymphatics.

Fig. 3

A. Schematic diagram of the intravascular injection procedure for filling blood vessels. B. Schematic diagram of the intracisternal injection procedure for filling intracranial lymphatics. C. Schematic diagram of the optic tecta, cerebellum, and intracranial lymphatics in the dorsal head of an adult casper mutant, Tg(mrc1a:egfp)y251 zebrafish. The red box notes the approximate area shown in the high-magnification images in panels D-L. D-F. Confocal images of intracranial blood vessels (BV) and lymphatic vessels (LV) in the dorsal head of a Tg(mrc1a:egfp)y251 adult fish injected intravascularly with Qdot705, showing mrc1a:egfp+ lymphatics and FGPs + Qdot705 (D), Qdot705 alone (E), or mrc1a:egfp+ alone (F). G-L. Confocal images of intracranial blood vessels (BV) and lymphatic vessels (LV) in the dorsal head of a Tg(mrc1a:egfp)y251, Tg(kdrl:mcherry) y206 adult fish injected intracranially with Qdot705 and blue dextran, showing mrc1a:egfp+ lymphatics and kdrl:mcherry+ blood vessels (G), kdrl:mcherry+ blood vessels (H), mrc1a:egfp+ lymphatics and blue dextran (I), blue dextran (J), mrc1a:egfp+ lymphatics and Qdot705 (K), Qdot705 (L). Scale bars: 50 um. See Online Video III for 3D renderings and real-time imaging of the vessels shown in panels G-L. (mrc1a- mannose receptor C, type 1a, FGPs- fluorescent granular perithelial cells, kdrl- kinase insert domain receptor like, BV- blood vessels, LV-lymphatic vessels)