Table 4.

Methylation associated with index variants showing significant statistical interaction with cranial radiation therapy (CRT) for effect on body mass index (BMI). CpGs were identified as associated with index variants across the lifecourse in ARIES then associated with BMI in adult survivors of childhood acute lymphoblastic leukemia in the CCSS diagnosed 1970-1986.

Locus	CRT Interaction Index SNP	CpG	CpG-SNP Distance	ARIES meQTL (CpG-SNP)			CCSS Cohort (CpG-Outcome)
				Timepoint	Effect	P	Methylation sample	Outcome	Effect	P
SRGAP3 intron	rs730901	cg24850121	−43,645	Middle Age	0.003	6.3E-08	Overall (n=96)	BMI	0.0013	0.18
							CRT treated (n=47)	BMI	−0.0005	0.8
							Not CRT treated (n=49)	BMI	0.0024	0.02
							Overall (n=96)	CRT	0.0057	0.6
UNCX-MICALL2	rs145623412	cg18423103	68,981	Pregnancy	0.058	2.1E-15	Overall (n=96)	BMI	0.0018	0.004
				Birth	0.002	5.9E-10	CRT treated (n=47)	BMI	0.0014	0.16
				Childhood	0.009	3.4E-18	Not CRT treated (n=49)	BMI	0.0019	0.02
				Adolescence	0.046	2.5E-19	Overall (n=96)	CRT	0.0015	0.8
				Middle Age	0.067	8.0E-16
UNCX-MICALL2	rs145623412	cg15701281	78,739	Pregnancy	0.017	1.7E-17	Overall (n=96)	BMI	−0.0009	0.17
				Birth	0.023	1.9E-16	CRT treated (n=47)	BMI	0.0012	0.27
				Childhood	0.008	1.3E-11	Not CRT treated (n=49)	BMI	−0.0021	0.006
				Adolescence	0.022	4.5E-15	Overall (n=96)	CRT	0.0006	0.9
				Middle Age	0.019	8.2E-12

BMI, body mass index; CpG, cytosine-guanine dinucleotide; CRT, cranial radiation therapy; meQTL, methylation quantitative trait locus; SNP, single nucleotide polymorphism.