Table 2 -.

mRNA expression of tight junctions in infant (2 week old) and adult (12 week old) mice intestines expressed as relative percentage of β actin.

Gene	Proximal small intestine		P value	Distal small intestine		P value	Colon		P value
Gene	Infant	Adult	P value	Infant	Adult	P value	Infant	Adult	P value
CLDN1	0.016 ± 0.004	0.01 ± 0.002	0.251	0.009 ± 0.002	0.011 ± 0.003	0.53	0.029 ± 0.006	0.052 ± 0.017	0.208
CLDN2	0.026 ± 0.007	0.004 ± 0.002	0.004^**	0.034 ± 0.018	0.009 ± 0.003	0.145	0.036 ± 0.022	0.028 ± 0.007	0.725
CLDN3	0.546 ± 0.039	0.987 ± 0.079	0.0003^***	0.312 ± 0.055	1.346 ± 0.143	0.0002^***	0.874 ± 0.125	5.042 ± 1.424	0.021^*
CLDN4	0.479 ± 0.099	0.155 ± 0.013	0.0073^**	0.447 ± 0.041	0.259 ± 0.064	0.056	0.303 ± 0.036	0.44 ± 0.149	0.424
CLDN5	0.091 ± 0.01	0.018 ± 0.002	<0. 0001^***	0.032 ± 0.004	0.035 ± 0.008	0.802	0.075 ± 0.019	0.122 ± 0.039	0.328
CLDN7	1.242 ± 0.053	1.617 ± 0.102	0.0115^*	0.856 ± 0.061	1.831 ± 0.241	0.009^**	1.024 ± 0.132	4.252 ± 1.009	0.008^**
CLDN8	0.202 ± 0.098	0.236 ± 0.082	0.795	1.672 ± 0.301	0.751 ± 0.241	0.036^*	1.633 ± 0.154	7.263 ± 3.373	0.153
CLDN12	0.021 ± 0.009	0.005 ± 0.001	0.113	0.012 ± 0.001	0.006 ± 0.002	0.354	0.072 ± 0.046	0.021 ± 0.003	0.327
CLDN13	0.038 ± 0.025	0.009 ± 0.002	0.209	0.016 ± 0.011	0.025 ± 0.008	0.509	0.035 ± 0.021	0.049 ± 0.016	0.613
CLDN15	0.543 ± 0.074	1.164 ± 0.202	0.013^*	0.169 ± 0.019	1.496 ± 0.591	0.109	0.243 ± 0.036	1.775 ± 0.634	0.05^*
ZO1	1.04 ± 0.101	3.132 ± 0.414	0.0003^***	0.955 ± 0.086	2.966 ± 0.301	0.0004^***	1.189 ± 0.142	4.535 ± 0.232	<0.0001^***
ZO2	0.019 ± 0.008	0.029 ± 0.003	0.266	0.007 ± 0.001	0.064 ± 0.021	0.062	0.018 ± 0.002	0.07 ± 0.03	0.118
ZO3	0.072 ± 0.015	0.376 ± 0.056	0.0002^***	0.044 ± 0.004	0.469 ± 0.111	0.013^*	0.048 ± 0.01	0.421 ± 0.099	0.004^**
OCLN	0.433 ± 0.063	0.853 ± 0.127	0.012^*	0.323 ± 0.032	1.11 ± 0.23	0.023^*	0.336 ± 0.049	1.401 ± 0.41	0.017^*
JAMA	0.846 ± 0.175	1.868 ± 0.331	0.018^*	0.68 ± 0.089	1.984 ± 0.327	0.008^**	0.835 ± 0.101	2.154 ± 0.397	0.005^**

Values that are significantly higher are in bold, n=6, Student’s unpaired t test:

P < 0.05

^**

P < 0.01

^***

P < 0.001.