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. 2020 Sep 5;29(1):291–307. doi: 10.1016/j.ymthe.2020.08.018

Figure 1.

Figure 1

NONO Correlates with LN Metastasis and Is a Prognostic Marker in BCa

(A) NONO expression was analyzed in acute myeloid leukemia (AML) (n = 10), B cell acute lymphoblastic leukemia (BCALL) (n = 10), bladder cancer (BLCA) (n = 11), breast cancer (BRCA) (n = 12), colorectal adenocarcinoma (COAD) (n = 12), diffuse large B cell lymphoma (DLBC) (n = 11), endometrial adenocarcinoma (ENAD) (n = 10), follicular lymphoma (FOLY) (n = 11), glioblastoma (GBM) (n = 10), lung adenocarcinoma (LUAD) (n = 12), medulloblastoma (MBM) (n = 10), melanoma (MEM) (n = 10), ovarian cancer (OVCA) (n = 12), pancreatic adenocarcinoma (PAAD) (n = 11), pleural mesothelioma (PLMA) (n = 11), prostate cancer (PRCA) (n = 14), renal cell carcinoma (RCCA) (n = 11), and T cell acute lymphoblastic leukemia (TBALL) (n = 10). The red line represents the mean value of the NONO expression in BCa. (B) NONO expression was analyzed between BCa tissues (n = 11) and bladder normal tissues (BNTs, n = 7). (C) Representative IHC images of the NONO expression in the paraffin-embedded NAT and tumor sections of BCa, with or without LN metastasis. Scale bars: black, 200 μm; red, 50 μm. (D) IHC staining of cohort 1 shows the NONO expression among NAT (n = 35), LN-negative (n = 84), and LN-positive (n = 29) tumor tissues. (E) NONO expression was analyzed between NMIBC (n = 54) and MIBC (n = 59) tissues in cohort 1. (F–H) NONO mRNA expression was analyzed between LN-negative (n = 4) versus LN-positive (n = 13) tissues in the Als bladder cohort (F), NMIBC (n = 28) versus MIBC (n = 81) in the Sanchez-Carbayo cohort (G), and NMIBC (n = 28) versus MIBC (n = 13) in the Dyrskjot bladder cohort (H). (I and J) Kaplan-Meier curves for the OS (I) and DFS (J) of BCa patients with high versus low expression of NONO in cohort 1. Patients were divided into the NONO-low (n = 55) and NONO-high (n = 58) groups. (K) Kaplan-Meier curves for OS of BCa patients with high (n = 293) versus low (n = 111) expression of NONO in the TCGA cohort. Statistical significance was assessed using two-tailed t tests or one-way analysis of variance (ANOVA). ∗p < 0.05, ∗∗p < 0.01.