Figure 6.

Restoration of SETMAR-L Reverses the Pro-metastasis Effects of the NONO Knockdown

(A) Relative expression of SETMAR-L was detected by qPCR after T24 and UM-UC-3 cells were treated as indicated. (B) NONO expression was analyzed by western blot after T24 and UM-UC-3 cells were treated as indicated. (C–E) Representative images of migrated (D) and invaded (E) cells, and the quantification (C) after T24 and UM-UC-3 cells were treated as indicated. (F) Kaplan-Meier survival analysis of mice inoculated with UM-UC-3 cell treatment as indicated. (G and H) Representative images of dissected popliteal LNs (G) and histogram analysis of the LN volume (H). (I) Representative images of the bioluminescence and H&E staining, confirming the LN status. The red arrows show the footpad tumor and metastatic popliteal LN. Scale bars: black, 500 μm; red, 100 μm. (J) Histogram analysis of bioluminescence from the treated nude mice as indicated (n = 8 per group). (K) Percentage of LN status in all groups. Statistical significance was assessed using a one-way ANOVA, followed by a Dunnett’s test. Error bars represent the standard deviations of three independent experiments. ∗p < 0.05, ∗∗p < 0.01.