Figure 6.

Nox4 Inhibition Decreased Amyloid Levels and Oxidative Stress in the Hippocampus of APP/PS1 Mice

(A and B) Representative image of Aβ staining in the hippocampus of GLX351322-treated APP/PS1 mice and quantification of the area percentage of Aβ plaque load. n = 10 slices from three mice in each group. Scale bars: 250 μm. (C and D) The levels of TBS-soluble, TBS-T-soluble, and FA-soluble Aβ_1–40 (C) and Aβ_1–42 (D) were determined by ELISA in the hippocampus of GLX351322-treated APP/PS1 mice. n = 3. (E and F) The levels of APP and secretases in the hippocampus were examined by western blotting, and quantification of the intensities normalized to GAPDH as a loading control was shown. n = 3. (G) The level of ROS in the hippocampus of GLX351322-treated APP/PS1 mice was detected by fluorescence. Scale bars: 250 μm. (H) The level of H₂O₂ in the hippocampus of GLX351322-treated APP/PS1 mice was examined by spectrophotometry. n = 5. (I–K) The levels of 4-HNE (I), 3-NT (J), and 8-OHdG (K) in the hippocampus of GLX351322-treated APP/PS1 mice were measured by ELISA. n = 5–6. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. ns, no significance.