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. 2021 Jan 7;12(1):43. doi: 10.1038/s41419-020-03295-y

Fig. 3. The increased vulnerability to MI/RI in diabetes is associated with HDAC3 mediated disruption of circadian gene expression oscillations by inhibiting mitophagy.

Fig. 3

A–C The mRNA levels of HDAC3 (A), Rev-erbα (B), and BMAL1 (C) were analysed by qPCR. D–G The protein levels of HDAC3 (E), Rev-erbα (F), and BMAL1 (G) were analysed by western blotting in myocardial tissues of non-diabetic and diabetic rats with I/R insult. H HE staining of hearts from NS, NI/R, DS, or DI/R mice at ZT12. Scale bar: 100 μm. I–O The protein levels of HDAC3 (J), Rev-erbα (K), BMAL1 (L), C/EBPβ (M), BNIP3 (N) and LC3 II/I (O) in NS, NI/R, DS or DI/R group at ZT12 were detected by western blotting. n = 6 per group. *P < 0.05 versus NI/R within ZT; #P < 0.05 versus ZT0 within NI/R; &P < 0.05 versus ZT0 within DI/R; △P < 0.05 versus NS; ◇P < 0.05 versus DS; ◆P < 0.05 versus NI/R.