Fig. 4. Genetic knockdown of HDAC3 recovered mitophagy status through the Rev-erbα/BMAL1 circadian pathway and significantly attenuated diabetic MI/RI.

A Representative images of myocardial tissue GFP and DAPI was detected by immunofluorescence staining after AAV-GFP and AAV-HDAC3 transfection, scale bar = 100 μm. B Protein expression of HDAC3 was analysed by western blotting. C Infarct size was measured by TTC. D The serum level of cTn-I (D) was measured by ELISA assay kits. E–G The mRNA expression of HDAC3 (E), Rev-erbα (F), and BMAL1 (G) were analysed by qPCR. H–O The protein levels of HDAC3 (I), Rev-erbα (J), BMAL1 (K), C/EBPβ (L), BNIP3 (M), P62 (N), and LC3 II/I (O) were measured by western blotting. n = 6 per group. ^**P < 0.01 versus DM; ^*P < 0.05 versus AAV-GFP+sham; ^#P < 0.05 versus AAV-GFP⁺I/R.