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. 2021 Jan 7;12(1):43. doi: 10.1038/s41419-020-03295-y

Fig. 6. Knockdown of HDAC3/Rev-erbα expression attenuated neonatal rat cardiomyocytes H/R injury by upregulating BMAL1 expression to activate mitophagy under HG condition.

Fig. 6

A Cell viability was analysed by CCK-8 kit. B The serum level of LDH was analysed by ELISA kit. C and D Mitochondrial ROS (C) and JC-1 (D) were detected to analyse mitochondrial function. Scale bar: 100 μm. E Tandem fluorescent mRFP-GFP-LC3 adenovirus was used to detect autophagic flux. Scale bar: 20 μm. F MitoTracker Green and LysoTracker Red staining were used to detect mitophagosome formation. Scale bar: 20 μm. G–I The mRNA levels of HDAC3 (G), Rev-erbα (H), and BMAL1 (I) were analysed by qPCR. J–P The protein levels of HDAC3 (K), Rev-erbα (L), BMAL1 (M), C/EBPβ (N), P62 (O), and LC3 II/I (P) were analysed by western blotting in the cultured neonatal rat cardiomyocytes. n = 6 per group. #P < 0.05 versus H/R.