Fig. 2. SCEL promotes GBC cell proliferation and tumor growth.

A The indicated GBC cells were transfected with small interfering RNA or SCEL gene plasmid to knock down or overexpress SCEL, respectively. Western blotting was performed to verify these cell lines, grayscale statistics at the bottom of Western blot band. B, C The colony formation was assessed in GBC cells and statistical significance was analyzed based on the numbers of colonies. D The proliferation of treated GBC cells was measured by CCK8 assay. E Expression levels of SCEL were examined in EH-GB-1 cells transfected with Lv-EV and Lv-SCEL. F Above tumor cells were subcutaneously transplanted into nude mice to develop tumors (n = 6 for each group). G The tumor size was measured twice a week, and the tumor growth curve was generated based on the mean tumor volume. H The expression of Ki67 was detected, statistical analysis (unpaired test) of Ki-67 positive cell count number under the IHC picture; Bar: 100 um. Unpaired t tests were used in C, D, G, and H; data are shown as the mean value ± SD; *P < 0.05.