Fig. 5. SCEL influences the venous thromboembolism formation in tumor-bearing mice.

The indicated GBC cells were subcutaneously transplanted into nude mice to develop tumors. Tumors were grown for 8 weeks. Venous thrombosis in mice was induced by ligation of the inferior vena cava (IVC). A The numbers of neutrophils in blood. B–C Level of IL8 concentration (B) and MPO–DNA (C) in the plasma from the different groups of mice. D Detection of NETs by immunofluorescence in tumor. E Clots were embedded in paraffin, sectioned longitudinally, and stained with hematoxylin and eosin. F Clots were harvested at 48 h after IVC ligation and weighed. G The indicated subcutaneously tumor mice received DNase I (50 Unit/mouse) 1 h before inferior vena cava (IVC) stasis and clot were collected at 48 h and weighed. *p < 0.05, **p < 0.001, ***p < 0.001.