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. 2021 Jan 4;12(1):31. doi: 10.1038/s41419-020-03300-4

Fig. 3. ZFPM2-AS1 depletion suppressed cell growth and promoted cell apoptosis in HCC.

Fig. 3

A HepG2 and SMMC-7721 cells were infected with shRNA lentivirus specifically targeting ZFPM2-AS1 (sh-ZFPM2-AS1-1, sh-ZFPM2-AS1-2), and the shRNA-knockdown efficiency was evaluated by qPCR analyses. B The proliferation of HCC cells after infection was determined by CCK-8 assays. C, D EdU assays examined the proliferative cells (red). DAPI was used to stain the cellular nuclei (blue). Scale bar = 200 μm. E, F Colony formation assays. G Flow cytometry analyzed the cell cycle of HCC cells after infection. H Cell apoptosis was determined by flow cytometry. Data are presented as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01.