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. 2021 Jan 4;12(1):33. doi: 10.1038/s41419-020-03306-y

Fig. 4. Screen the potential targets of miR-153-5p and AGO1 was identified as its direct target.

Fig. 4

A Schematic illustration displaying the overlapped target genes of miR-153-5p predicted by microT-CDS, TargetScan, miRWalk, miRPathDB, and miRDB. B The mRNA expression of the top target genes were tested by qPCR in 786-O and TK10 after transfection with miR-153-5p mimics. C Sequence alignment of the predicted miR-153-5p-binding targets in the AGO1 3′-UTR from six organisms. D The protein expression of AGO1 was tested in 786-O and TK10 after transfection with miR-153-5p mimics by western blotting. E The sequence of binding targets of miR-153-5p in wild-type or mutant AGO1 3′-UTR. F After transfection with miR-NC or miR-153-5p in HEK293T cells, the relative luciferase activity of mutant or wild-type AGO1 3′-UTR was detected. All experiments were conducted at least three independent times and all the values were presented as mean ± SEM. *P < 0.05, **P < 0.01.