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. 2021 Jan 7;12(1):11. doi: 10.1038/s41419-020-03353-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Experimental design for examining the effect of serum factors on mature human myotubes. b Serum-treated myotubes were stained for MyHC (left) and the MyHC-positive area was quantified (right). n = 8 or 6 independent wells for 5% or 10% serum treatment, respectively. Data represent individual data points and the means. Data were analysed using the two-sided unpaired t-test. Scale bar, 100 μm (b).