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. 2021 Jan 7;12(1):11. doi: 10.1038/s41419-020-03353-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Serum levels of TNFα at day 3, 5, and 7 post-operation were measured using ELISA. b The expression level of Tnf was quantified in the liver (left) and skeletal muscle (right) at day 7 post-operation. c Liver sections of Pdgfrα^EGFP mice at day 21 post-operation were subjected to immunofluorescence staining for collagen I and DAPI. d A representative FACS plot for nuclei isolation. Nuclei were isolated from freshly frozen fibrotic livers of Pdgfrα^EGFP mice using FACS. Three gates were set as indicated in the plot to isolate EGFP⁺, EGFP⁻ diploid, and EGFP⁻ tetraploid nuclei. e The expression levels of hepatic stellate cell markers (Pdgfra and Lrat) and fibrosis markers (Acta2 and Col1a1) were quantified. f The expression levels of the hepatocyte marker (Hnf4a), Kupffer cell marker (Adgre1), and endothelial cell marker (Kdr) were quantified. g Quantitative analysis of Tnf gene expression. h The expression levels of Fbxo32 and Trim63 in the skeletal muscle were quantified. i The expression levels of Mstn in the skeletal muscle were quantified. n = 5, 6, and 9 (sham) and 5, 8, and 12 (BDL) mice for day 3, 5, and 7, respectively (a), n = 7 (sham) and 8 (BDL) mice for the liver, n = 7 (sham) and 9 (BDL) mice for the skeletal muscle (b, h, i), and n = 5 mice (e–g). Data represent individual data points and the means. Data were analysed using the two-sided unpaired t-test, t-test with Welch’s correction, or Kruskal–Wallis non-parametric test followed by Dunn’s post hoc test. NS not significant, ND not detected. Scale bar, 100 μm (c).