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. 2021 Jan 7;12(1):48. doi: 10.1038/s41419-020-03280-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blot (WB, upper) and RT-PCR (lower) analysis of CXCR4 in A549/GR cells transfected with shControl (pGFP) or shCXCR4A and D. B IR clonogenic assay of A549/GR cells transfected with shCXCR4D. Cells (2.5 × 10²) were exposed to IR at various doses described in the Figure. IR-exposed cells were evaluated colony-forming activity at 10 days after IR. *P < 0.05, **P < 0.01, ***P < 0.005. C Western blot analysis of A549/GR cells exposed to IR at 6 Gy at the indicated time points transfected with shControl (pGFP) or shCXCR4D. D γ-H2AX foci assay of A549/GR cells. ICC was performed with the cells transfected with shControl (pGFP) or shCXCR4D using γ-H2AX antibody after exposing to IR (6 Gy) at the indicated time points. Representative immunofluorescence images of A549/GR cells (left) and quantification of the assay (right). Nuclei were counterstained with DAPI (blue). White bar: 20 μm.