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. 2021 Jan 7;12(1):48. doi: 10.1038/s41419-020-03280-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Western blot (WB, upper) and RT-PCR (lower) analysis of NSCLC cells (A549 and H460) transfected with CXCR4. β-actin and GAPDH were used as a loading control for Western blot and RT-PCR, respectively. B IR clonogenic survival assay of control vector (pQCXIP) and CXCR4 overexpressing (CXCR4) NSCLC cells. Cells were exposed to IR at the indicated dose in the Figure. C γ-H2AX foci assay of A549 (upper) and H460 (lower) cells. Cells were transfected either with control vector (pQCXIP) or pQCXIP-CXCR4 (CXCR4) and were exposed to IR (6 Gy) and fixed at the indicated time points in the Figure. Representative ICC images of γ-H2AX (left) and quantification of the results (right). Nuclei were counterstained with DAPI (blue). White bar: 20 μm. *P < 0.05, **P < 0.01. D Western blot analysis of A549 (left) and H460 (right) cells exposed to IR (6 Gy) at the indicated time points transfected with control vector (pQCXIP) or pQCXIP-CXCR4 (CXCR4). H2AX and β-actin were used as loading controls.