Skip to main content
. 2021 Jan 6;7:1. doi: 10.1038/s41420-020-00384-x

Fig. 4. Enhanced expression of Nogo-A and myogenic factors during myoblast differentiation.

Fig. 4

Cells were cultured in growth medium (GM) to maintain a proliferation/early differentiation state; differentiation was induced by incubating cells with differentiation medium (DM) for 3 days. A, B IF staining of Nogo-A (green) with Pax7, MyoD, myogenin, or MYH2 (red) in C2C12 mouse myoblast cells in early differentiation (A) and late differentiation (B). Scale bar = 20 µm. C Summary of the expression levels of Nogo-A and myogenic factors during myoblast differentiation. D qRT-PCR analysis of Nogo-A and myogenic factors in myoblasts (n = 3/group). Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. E qRT-PCR analysis of Nogo-A in induced muscle stem cells (iMSCs) and sorted-iMSCs (sort-iMSCs). F, G IF staining of Nogo-A (red) with Pax7 or MYH2 (green) in differentiated iMSCs (F) and sort-iMSCs (G). Scale bar = 20 µm.