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. 2021 Jan 4;12(1):5. doi: 10.1038/s41419-020-03296-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blot analysis was performed to detect ATF4 silencing efficiency. B EdU was applied to detect pancreatic acinar cell proliferation after sh-ATF4-1 or sh-ATF4-2 treatment. C TUNEL was performed to detect pancreatic acinar cell apoptosis after sh-ATF4-1 or sh-ATF4-2 treatment. D Flow cytometry was performed to detect pancreatic acinar cell apoptosis after sh-ATF4-1 or sh-ATF4-2 treatment. E Western blot analysis was performed to detect expression of Bcl-2, Bax and cleaved-caspase3. F ELISA was conducted to test levels of IL-1β, IL-6, TNF-α, and IL-10. *p < 0.05 compared with the sh-NC group. The comparison of the measurement data (mean standard ± deviation) among multiple groups was analyzed by one-way analysis of variance with Tukey’s post hoc test.