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. 2021 Jan 4;12(1):5. doi: 10.1038/s41419-020-03296-x

Fig. 7. Downregulation of ATF4 promotes pancreatic acinar cell proliferation via regulation of NEP-KLF4 expression by inhibiting HDAC1.

Fig. 7

A Western blot analysis was performed to detect ATF4, HDAC1, NEP, and KLF4. B EdU was applied to detect pancreatic acinar cell proliferation after sh-ATF4 or oe-KLF4 treatment. C TUNEL was performed to detect pancreatic acinar cell apoptosis after sh-ATF4 or oe-KLF4 treatment. D Flow cytometry was performed to detect pancreatic acinar cell apoptosis after sh-ATF4 or oe-KLF4 treatment. E Western blot analysis was performed to detect expression of Bcl-2, Bax, and cleaved-caspase3. F ELISA was conducted to test levels of IL-1β, IL-6, TNF-α, and IL-10. *p < 0.05 compared with the sh-NC + oe-NC group; #p < 0.05 compared with the sh-ATF4 + oe-NC group. The comparison of the measurement data (mean standard ± deviation) among multiple groups was analyzed by one-way analysis of variance with Tukey’s post hoc test.