Fig. 2. Impairment in TAG biosynthesis protects against α-syn overexpression.

A Cellular TAG content was measured with a detection kit in pcDNA and WT α-syn cells. B [³H]-OA incorporation into MAG, DAG and TAG, and FFA release were measured in our model. C DGAT2 upregulation in cells overexpressing α-syn was determined by Western blot and RT-qPCR. D ACS activity was studied by the measurement of [³H] oleyl -CoA formation in pcDNA and WT α-syn neurons. E TAG lipase activity was determined through the detection of the products of TAG hydrolysis in a radioactivity assay. F The role of TAG biosynthesis during α-syn overexpression was determined in cells treated with the lipin-1 inhibitor DL-propranolol by the MTT reduction assay. A–F All experiments were repeated three times. Bars represent means ± standard deviation (SD, n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 with respect to the control conditions.