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. 2020 Nov 24;54(1):e12957. doi: 10.1111/cpr.12957

Figure 4.

Figure 4

LINC01126 serves as a sponge for miR‐518. (A) Venn diagrams showing the number of potential miRNAs targeting LINC01126 (including miR‐518a‐5p). The potential miRNAs were predicted by four databases: LncBase, miRcode, Starbase, StarBaseV2. (B) Fluorescence in situ hybridization results demonstrated the subcellular location of INC01126 and miR‐518. Nuclei were stained by DAPI (blue), the scale bar = 50 μm. (C) The predicted binding of miR‐518a‐5p with LINC01126 3′‐UTR. The sequence of wild‐type (WT) and mutant (Mut) LINC01126 target sites were shown. (D) RNA immunoprecipitation assay of endogenous AGO2 binding to RNA in hPDLCs. IgG was used as the negative control. The expression of LINC01126 and miR‐518a‐5p was analysed by qRT‐PCR and the results were normalized relative to the input control. (E) Dual‐luciferase reporter assay validating the interaction between miR‐518a‐5p and LINC01126. (F) The expression of miR‐518a‐5p after transfection with pCDH‐01126, sh‐01126 and the corresponding controls. (G) The expression of miR‐518a‐5p after transfection with miR‐518a‐5p mimic, miR‐518a‐5p‐inhibitor and the corresponding controls. Results are presented as mean ± SD (**P < .01, compared with the control group)