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. 2020 Nov 24;54(1):e12957. doi: 10.1111/cpr.12957

Figure 5.

Figure 5

HIF‐1α is a target of LINC01126/ miR‐518a‐5p. (A) Relative expression of LINC01126 and in nuclear and cytoplasmic fractions of hPDLCs. (B) Venn diagrams showing the number of potential genes targeting miR‐518a‐5p (including HIF‐1α gene). The potential genes were predicted by three databases: TargetScan, miRWalk and miRTar. (C) The predicted binding of miR‐154‐5p with HIF‐1α 3ʹ UTR. (D) Dual‐luciferase reporter assay validating the interaction between miR‐518a‐5p and LINC01126. (E) The mRNA expression of HIF‐1α after transfection with miR‐518a‐5p mimic, miR‐518a‐5p inhibitor and the corresponding controls. (F) The mRNA expression of HIF‐1α after transfection with pCDH‐01126, LINC01126‐miR‐518a‐5p, sh‐01126, sh‐01126‐miR‐518a‐5p and the corresponding controls. (G) The protein expression level after transfection with pCDH‐01126, sh‐01126 and the corresponding controls. (H‐I) The protein expression of HIF‐1α after transfection with pCDH‐01126, sh‐01126 and the corresponding controls under different O2 condition. Histograms show quantification of the band intensities. Results are presented as mean ± SD (**P < .01, compared with the control group)