Skip to main content
. 2020 Dec;8(23):1586. doi: 10.21037/atm-20-3647

Table 1. Primary antibodies used.

Antigen Description of immunogen Source, host species, catalog no. Concentration used RRIDs
Anti-GAPDH GAPDH fusion protein Ag0766 Proteintech, rabbit polyclonal antibody, 10494-1-AP 1:10,000 (WB) AB_2263076
Anti-LC3 Residues surrounding Leu44 of human LC3B protein (conserved in LC3A) Cell Signalling Technology, rabbit monoclonal antibody, #12741 1:1,000 (WB) AB_2617131
Anti-p62 P62/SQSTM1 fusion protein Ag13131 Proteintech, rabbit polyclonal antibody, 18420-1-AP 1:1,000 (WB) AB_10694431
Anti-ATG5 Residues surrounding Leu265 of human Atg5 protein Cell Signaling Technology, rabbit monoclonal antibody, #12994 1:1,000 (WB) AB_2630393
Anti-beclin-1 Beclin 1 fusion protein Ag1843 Proteintech, rabbit polyclonal antibody, 11306-1-AP 1:1,000 (WB) AB_2259061
Anti-Sirt1 A recombinant protein representing the central region of human SirT1 protein Cell Signaling Technology, mouse monoclonal antibody, #8469 1:1,000 (WB) AB_10999470
Anti-cleaved-caspase 3 Synthesized peptide derived from human caspase 3 Affinity Biosciences, rabbit polyclonal antibody, AF7022 1:1,000 (WB) AB_2835326
Anti-cleaved-PARP Synthetic peptide within human cleaved PARP1 aa 150–250 Abcam, rabbit monoclonal antibody, ab32064 1:1,000 (WB) AB_777102
FoxO1 Synthetic peptide mapping to a region between residues 600 and the C-terminus (residue 652) of human FOXO1A Abcam, rabbit polyclonal antibody, ab70382 1:500 (IF), 1:1,000 (WB) AB_1268912
Anti-acetylated-lysine A synthetic acetylated lysine-containing peptide Cell Signalling Technology, rabbit antibody, #9441 1:1,000 (WB) AB_331805
Anti-histone-H3 Histone-H3 fusion protein Ag10644 Proteintech, rabbit polyclonal antibody, 17168-1-AP 1:3,000 (WB) AB_2716755
Anti-tubulin Beta-tubulin fusion protein Ag0117 Proteintech, rabbit polyclonal antibody, 10068-1-AP 1:3,000 (WB) AB_2303998