Fig. 1.

Effect of CR-31-B (−) on dual reporter gene expression from constructs containing different 5′-UTRs. (A) Structure of the synthetic rocaglate CR-31-B (−). (B) Schematic illustration of the dual luciferase reporter construct used to determine eIF4A-dependent translation of coronavirus 5′-UTRs. (C) Effects of 5 and 10 nM CR-31-B (−) on reporter gene expression in the context of 5′-UTRs from three human coronaviruses, HCoV-229E, MERS-CoV and SARS-CoV-2. The 5′-UTR of the human β-globin mRNA and the unstructured (AC)₁₅ sequence served as negative controls, while the (AG)₁₅ polypurine sequence served as a positive control. Experiments were performed using previously described protocols (Müller et al., 2018a, 2020) with at least three independent biological replicates.