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. 2021 Jan 6;220(2):e201911025. doi: 10.1083/jcb.201911025

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© 2020 Ma et al.

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Figure 1. — DSBs promote microtubule dynamics in G1 cells. (A) U2OS at G1/S phase were treated with 2 Gy IR, released, and fixed at indicated time points. Microtubules (green) were stained with anti–β-tubulin antibody in microtubule regrowth assay. Scale bar, 20 µm. (B) Quantitation of microtubule length after IR as assayed in A showed the rate of microtubule polymerization (n > 50). (C) Microtubule regrowth assay was performed in G1 MCF7 cells 4 h after IR. Scale bar, 20 µm. (D) Quantitation of microtubules length 4 h after treatment with different doses of IR in G1 MCF7 cells (n > 50). (E) Quantitation of microtubule length 4 h after 5 Gy IR treatment in G1 HeLa cells. (F) Quantitation of microtubule length after IR in G1 RPE-1 cells (n > 50). (G) The kinetics of DNA damage repair were determined by 53BP1 or γH2AX foci formation (n > 100). (H) Quantitation of microtubule length 4 h after indicated treatment in G1 U2OS cells (n > 50). (I) Quantitation of microtubule length 4 h after treatment with indicated doses of IR in G1 RPE-1 cells (n > 50). P values are from un-paired t test: ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant. Values are shown as mean ± SD in this and following experiments. MT, microtubule; UT, untreated.