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. 2020 Dec 1;13(12):3033–3043.

Figure 1.

Figure 1

Expression and amplification of NCF2 in ESCC cell lines. A. The level of NCF2 protein examined by Western blotting in 8 human HCC cell lines (i.e., K30, Eca109, TE1, HK, K180, K510 and K520) and one immortalized primary hepatocellular epithelial cell line NE1. B. The expression level of NCF2 mRNA in 8 human ESCC cell lines was evaluated by q-PCR. C. Among 10 ESCC cases, increased expression of NCF2 was detected via western blotting in 8 pairs of ESCC tissues compared with the matched non-cancerous tissues. The expression levels were normalised to those of NCF2. D. The mRNA expression of NCF2 was significantly up-regulated in 8/10 pairs of HCC tissues based on q-PCR. The expression levels were normalised to those of GAPDH. (N, adjacent normal tissue; T, ESCC tissue).