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CXCL5 is a direct target of miR-77. Based on online bioinformatics software, miR-577 can bind with the 3’-UTR of CXCL5 (A). A luciferase reporter gene was constructed containing either a CXCL5 WT-type or Mut-type 3’-UTR of the miR-577 binding site (B). After transfection with miR-577 mimics or miR-577-Mut sequence into Hela cells, RT-qPCR (C) and western blot (D and E) were performed to measure CXCL5 mRNA and protein expression. n = 3 in each group. *P < 0.05.
